Reflections and Images

     I am sitting at my desk in the lab, still mad and frustrated that I can’t even pipette properly. My cell counter results are dismal. I don’t know why I am getting such poor percentages for cell viability – I should be getting at least 90%, not 47%. I followed what my supervisor did an hour ago: perform a serial dilution four times, pipette trypan blue into each of the four wells, then pipette a bit of sample into the cell counter plate.       Fortunately, my supervisor came into the cell room to check on me and helped me with pipetting for the cell counter step. He got 89%, close to the ideal threshold. “I don’t know what’s going on,” he said. I could sense that his voice was tinged with frustration, which I understood. I was also mad for not having an answer. What exactly was wrong with my pipetting technique? Did I pipette too quickly, or did I not press the plunger correctly?       The whole cell counter incident frustrates ...